› 6crk

X-ray diffraction
2Å resolution

Heterotrimeric G-protein in complex with an antibody fragment

Released:
DOI: 10.2210/pdb6crk/pdb
PDB entry 6crk coloured by chain, front view.
PDB entry 6crk coloured by chain, side view.
PDB entry 6crk coloured by chain, top view.
Source organisms:
Primary publication:
Development of an antibody fragment that stabilizes GPCR/G-protein complexes.
Maeda S, Koehl A, Matile H, Hu H, Hilger D, Schertler GFX, Manglik A, Skiniotis G, Dawson RJP, Kobilka BK
Nat Commun 9 3712 (2018)
PMID: 30213947

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-230946 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63096 (Residues: 2-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
single-chain Fv Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P2221
Unit cell:
a: 58.514Å b: 104.739Å c: 211.821Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.21
Expression systems:
  • Escherichia coli
  • Trichoplusia ni

Quick links

Citations

11 review citations

Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology.
El Khamlichi et al. (2019)
10 more

12 mentions without citation

Every Detail Matters. That Is, How the Interaction between Gα Proteins and Membrane Affects Their Function.
Polit et al. (2021)
11 more