› 6cw2

X-ray diffraction
2.67Å resolution

Crystal structure of a yeast SAGA transcriptional coactivator Ada2/Gcn5 HAT subcomplex, crystal form 1

Released:
DOI: 10.2210/pdb6cw2/pdb
PDB entry 6cw2 coloured by chain, front view.
PDB entry 6cw2 coloured by chain, side view.
PDB entry 6cw2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S
Proc Natl Acad Sci U S A 115 10010-10015 (2018)
PMID: 30224453

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-211894 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone acetyltransferase GCN5 Chain: D
Molecule details ›
Chain: D
Length: 251 amino acids
Theoretical weight: 29.08 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03330 (Residues: 67-317; Coverage: 57%)
Gene names: ADA4, GCN5, SWI9, YGR252W
Sequence domains: Acetyltransferase (GNAT) family
Transcriptional adapter 2 Chain: C
Molecule details ›
Chain: C
Length: 120 amino acids
Theoretical weight: 13.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02336 (Residues: 1-120; Coverage: 28%)
Gene names: ADA2, D9461.33, YDR448W
Sequence domains:
antibody heavy chain Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
antibody light chain Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 191.816Å b: 191.816Å c: 92.674Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.25 0.284
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

6 review citations

The SAGA chromatin-modifying complex: the sum of its parts is greater than the whole.
Soffers et al. (2020)
5 more

3 mentions without citation

The Ada2/Ada3/Gcn5/Sgf29 histone acetyltransferase module.
Espinola-Lopez et al. (2021)
2 more