› 6cz5

X-ray diffraction
3Å resolution

Crystal structure of small molecule AMP-acrylamide covalently bound to DDX3 S228C

Released:
DOI: 10.2210/pdb6cz5/pdb
PDB entry 6cz5 coloured by chain, front view.
PDB entry 6cz5 coloured by chain, side view.
PDB entry 6cz5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Chemical genetic inhibition of DEAD-box proteins using covalent complementarity.
Barkovich KJ, Moore MK, Hu Q, Shokat KM
Nucleic Acids Res 46 8689-8699 (2018)
PMID: 30102385

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX3X Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 54.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00571 (Residues: 132-607; Coverage: 72%)
Gene names: DBX, DDX3, DDX3X
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FPJ 1 x FPJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 53.96Å b: 101.09Å c: 105.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

RNA Helicases as Shadow Modulators of Cell Cycle Progression.
Sergeeva et al. (2021)
2 more

5 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
4 more