› 6czz

X-ray diffraction
1.7Å resolution

Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP-phosphoserine geminal diamine intermediate

Released:
DOI: 10.2210/pdb6czz/pdb
PDB entry 6czz coloured by chain, front view.
PDB entry 6czz coloured by chain, side view.
PDB entry 6czz coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana- the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis.
Sekula B, Ruszkowski M, Dauter Z
Front Plant Sci 9 876 (2018)
PMID: 30034403

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase 1, chloroplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 362 amino acids
Theoretical weight: 40.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96255 (Residues: 72-430; Coverage: 84%)
Gene names: At4g35630, F8D20.140, PSAT1
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
Ligand SEP 4 x SEP
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 189.668Å b: 53.288Å c: 137.921Å
α: 90° β: 91.91° γ: 90°
R-values:
R R work R free
0.181 0.181 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural Basis for Allostery in PLP-dependent Enzymes.
Tran et al. (2022)
8 other articles

2 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
1 more