› 6d65

X-ray diffraction
2.35Å resolution

Crystal structure of the human dual specificity phosphatase 1 catalytic domain (C258S) as a maltose binding protein fusion in complex with the designed AR protein off7

Released:
DOI: 10.2210/pdb6d65/pdb
PDB entry 6d65 coloured by chain, front view.
PDB entry 6d65 coloured by chain, side view.
PDB entry 6d65 coloured by chain, top view.
Source organisms:
Primary publication:
MBP-binding DARPins facilitate the crystallization of an MBP fusion protein.
Gumpena R, Lountos GT, Waugh DS
Acta Crystallogr F Struct Biol Commun 74 549-557 (2018)
PMID: 30198887

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142529 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity protein phosphatase 1; Maltose/maltodextrin-binding periplasmic protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 520 amino acids
Theoretical weight: 57.1 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-391; Coverage: 99%)
  • Canonical: P28562 (Residues: 172-314; Coverage: 39%)
Gene names: CL100, DUSP1, JW3994, MKP1, PTPN10, VH1, b4034, malE
Sequence domains:
Designed AR protein off7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 169 amino acids
Theoretical weight: 18.31 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

3 bound ligands:
Ligand GOL 6 x GOL
Ligand SO4 23 x SO4
Ligand EOH 21 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 75.327Å b: 109.503Å c: 218.558Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Heterologous Expression of Toxic White Spot Syndrome Virus (WSSV) Protein in Eengineered Escherichia coli Strains.
Chen et al. (2023)

1 mention without citation

MBP-binding DARPins facilitate the crystallization of an MBP fusion protein.
Gumpena et al. (2018)