› 6d85

X-ray diffraction
2.2Å resolution

Structure of the Bovine p85a BH domain E217K mutant

Released:
DOI: 10.2210/pdb6d85/pdb
PDB entry 6d85 coloured by chain, front view.
PDB entry 6d85 coloured by chain, side view.
PDB entry 6d85 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Patient-derived mutations within the N-terminal domains of p85α impact PTEN or Rab5 binding and regulation.
Mellor P, Marshall JDS, Ruan X, Whitecross DE, Ross RL, Knowles MA, Moore SA, Anderson DH
Sci Rep 8 7108 (2018)
PMID: 29740032

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 3-kinase regulatory subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 21.91 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23727 (Residues: 110-302; Coverage: 27%)
Gene name: PIK3R1
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 85.474Å b: 91.647Å c: 93.556Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Conformationally active integrin endocytosis and traffic: why, where, when and how?
Mana et al. (2020)
3 more

3 mentions without citation

Patient-derived mutations within the N-terminal domains of p85α impact PTEN or Rab5 binding and regulation.
Mellor et al. (2018)
2 more