› 6dah

X-ray diffraction
2.5Å resolution

2.5 Angstrom crystal structure of the N97S CaM mutant

Released:
DOI: 10.2210/pdb6dah/pdb
PDB entry 6dah coloured by chain, front view.
PDB entry 6dah coloured by chain, side view.
PDB entry 6dah coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Arrhythmia mutations in calmodulin cause conformational changes that affect interactions with the cardiac voltage-gated calcium channel.
Wang K, Holt C, Lu J, Brohus M, Larsen KT, Overgaard MT, Wimmer R, Van Petegem F
Proc Natl Acad Sci U S A 115 E10556-E10565 (2018)
PMID: 30348784

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 151 amino acids
Theoretical weight: 17.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 16 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 59.117Å b: 94.414Å c: 60.977Å
α: 90° β: 94.42° γ: 90°
R-values:
R R work R free
0.221 0.218 0.279
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The Crossroad of Ion Channels and Calmodulin in Disease.
Urrutia et al. (2019)
3 more

1 mention without citation

Arrhythmia mutations in calmodulin cause conformational changes that affect interactions with the cardiac voltage-gated calcium channel.
Wang et al. (2018)