6ddl

X-ray diffraction
2.26Å resolution

Crystal structure of the single mutant (D52N) of NT5C2-Q523X in the basal state

Released:

Function and Biology Details

Reactions catalysed:
A nucleotide + a 2'-deoxynriboucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
IMP + H(2)O = inosine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-156076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic purine 5'-nucleotidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 541 amino acids
Theoretical weight: 62.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49902 (Residues: 1-523; Coverage: 93%)
Gene names: NT5B, NT5C2, NT5CP, PNT5
Sequence domains: 5' nucleotidase family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: C2
Unit cell:
a: 143.446Å b: 123.634Å c: 91.251Å
α: 90° β: 115.51° γ: 90°
R-values:
R R work R free
0.199 0.194 0.243
Expression system: Escherichia coli