› 6dnh

Electron Microscopy
3.4Å resolution

Cryo-EM structure of human CPSF-160-WDR33-CPSF-30-PAS RNA complex at 3.4 A resolution

Released:
DOI: 10.2210/pdb6dnh/pdb
PDB entry 6dnh coloured by chain, front view.
PDB entry 6dnh coloured by chain, side view.
PDB entry 6dnh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the recognition of the human AAUAAA polyadenylation signal.
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
Proc Natl Acad Sci U S A 115 E1419-E1428 (2018)
PMID: 29208711
Related structures: EMD-7112

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131972 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Cleavage and polyadenylation specificity factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1443 amino acids
Theoretical weight: 161.07 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q10570 (Residues: 1-1443; Coverage: 100%)
Gene names: CPSF1, CPSF160
Sequence domains:
pre-mRNA 3' end processing protein WDR33 Chain: B
Molecule details ›
Chain: B
Length: 587 amino acids
Theoretical weight: 67.55 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9C0J8 (Residues: 1-572; Coverage: 43%)
Gene names: WDC146, WDR33
Sequence domains: WD domain, G-beta repeat
Cleavage and polyadenylation specificity factor subunit 4 Chain: C
Molecule details ›
Chain: C
Length: 245 amino acids
Theoretical weight: 27.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95639 (Residues: 1-269; Coverage: 91%)
Gene names: CPSF30, CPSF4, NAR, NEB1
Sequence domains:
RNA (5'-R(P*AP*AP*UP*AP*AP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.36 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-7112
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

20 review citations

Alternative cleavage and polyadenylation in health and disease.
Gruber et al. (2019)
19 more

5 mentions without citation

3'-End Processing of Eukaryotic mRNA: Machinery, Regulation, and Impact on Gene Expression.
Boreikaitė et al. (2023)
4 more