› 6e4r

X-ray diffraction
2.06Å resolution

Crystal Structure of the Drosophila Melanogaster Polypeptide N-Acetylgalactosaminyl Transferase PGANT9B

Released:
DOI: 10.2210/pdb6e4r/pdb
PDB entry 6e4r coloured by chain, front view.
PDB entry 6e4r coloured by chain, side view.
PDB entry 6e4r coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
A molecular switch orchestrates enzyme specificity and secretory granule morphology.
Ji S, Samara NL, Revoredo L, Zhang L, Tran DT, Muirhead K, Tabak LA, Ten Hagen KG
Nat Commun 9 3508 (2018)
PMID: 30158631

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-galactosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative polypeptide N-acetylgalactosaminyltransferase 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 507 amino acids
Theoretical weight: 57.94 KDa
Source organism: Drosophila melanogaster
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q8MRC9 (Residues: 146-650; Coverage: 77%)
Gene names: CG30463, Pgant9
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand EDO 7 x EDO
Ligand GOL 8 x GOL
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Unit cell:
a: 113.913Å b: 48.44Å c: 232.037Å
α: 90° β: 91.4° γ: 90°
R-values:
R R work R free
0.166 0.164 0.207
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials.
Ismaya et al. (2020)
5 more

3 mentions without citation

Polypeptide GalNAc-Ts: from redundancy to specificity.
de Las Rivas et al. (2019)
2 more