› 6ehr

X-ray diffraction
2.9Å resolution

The crystal structure of the human LAMTOR-RagA CTD-RagC CTD complex

Released:
DOI: 10.2210/pdb6ehr/pdb
PDB entry 6ehr coloured by chain, front view.
PDB entry 6ehr coloured by chain, side view.
PDB entry 6ehr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human lysosomal mTORC1 scaffold complex and its impact on signaling.
de Araujo MEG, Naschberger A, Fürnrohr BG, Stasyk T, Dunzendorfer-Matt T, Lechner S, Welti S, Kremser L, Shivalingaiah G, Offterdinger M, Lindner HH, Huber LA, Scheffzek K
Science 358 377-381 (2017)
PMID: 28935770

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128946 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Ragulator complex protein LAMTOR3 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UHA4 (Residues: 1-124; Coverage: 100%)
Gene names: LAMTOR3, MAP2K1IP1, MAPKSP1, PRO2783
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Structure domains: Dynein light chain 2a, cytoplasmic
Ragulator complex protein LAMTOR2 Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 13.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2Q5 (Residues: 2-125; Coverage: 99%)
Gene names: HSPC003, LAMTOR2, MAPBPIP, ROBLD3
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic
Ragulator complex protein LAMTOR5 Chain: C
Molecule details ›
Chain: C
Length: 91 amino acids
Theoretical weight: 9.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43504 (Residues: 1-91; Coverage: 100%)
Gene names: HBXIP, LAMTOR5, XIP
Sequence domains: Ragulator complex protein LAMTOR5
Structure domains: Dynein light chain 2a, cytoplasmic
Ragulator complex protein LAMTOR4 Chain: D
Molecule details ›
Chain: D
Length: 99 amino acids
Theoretical weight: 10.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q0VGL1 (Residues: 1-99; Coverage: 100%)
Gene names: C7orf59, LAMTOR4
Ragulator complex protein LAMTOR1 Chain: E
Molecule details ›
Chain: E
Length: 145 amino acids
Theoretical weight: 15.83 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6IAA8 (Residues: 21-161; Coverage: 88%)
Gene names: C11orf59, LAMTOR1, PDRO, PP7157
Sequence domains: Late endosomal/lysosomal adaptor and MAPK and MTOR activator
Ras-related GTP-binding protein A Chain: F
Molecule details ›
Chain: F
Length: 132 amino acids
Theoretical weight: 15.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7L523 (Residues: 183-313; Coverage: 42%)
Gene name: RRAGA
Structure domains: Beta-Lactamase
Ras-related GTP-binding protein C Chain: G
Molecule details ›
Chain: G
Length: 163 amino acids
Theoretical weight: 18.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HB90 (Residues: 239-399; Coverage: 40%)
Gene name: RRAGC
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 87.09Å b: 92.448Å c: 127.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.263
Expression system: Spodoptera frugiperda

Quick links

Citations

18 review citations

Lysosomes as dynamic regulators of cell and organismal homeostasis.
Ballabio et al. (2020)
17 more

10 mentions without citation

Increased Serine and One-Carbon Pathway Metabolism by PKCλ/ι Deficiency Promotes Neuroendocrine Prostate Cancer.
Reina-Campos et al. (2019)
9 more