› 6ej5

X-ray diffraction
3.34Å resolution

A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner

Released:
DOI: 10.2210/pdb6ej5/pdb
PDB entry 6ej5 coloured by chain, front view.
PDB entry 6ej5 coloured by chain, side view.
PDB entry 6ej5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner.
Gowravaram M, Bonneau F, Kanaan J, Maciej VD, Fiorini F, Raj S, Croquette V, Le Hir H, Chakrabarti S
Nucleic Acids Res 46 2648-2659 (2018)
PMID: 29378013

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nonsense transcripts 1 Chain: A
Molecule details ›
Chain: A
Length: 637 amino acids
Theoretical weight: 71.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92900 (Residues: 295-925; Coverage: 56%)
Gene names: KIAA0221, RENT1, UPF1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P213
Unit cell:
a: 146.852Å b: 146.852Å c: 146.852Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.286 0.284 0.309
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Nonsense-mediated mRNA decay: The challenge of telling right from wrong in a complex transcriptome.
Kishor et al. (2019)
7 more

3 mentions without citation

A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner.
Gowravaram et al. (2018)
2 more