› 6ejl

X-ray diffraction
2.38Å resolution

Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif

Released:
DOI: 10.2210/pdb6ejl/pdb
PDB entry 6ejl coloured by chain, front view.
PDB entry 6ejl coloured by chain, side view.
PDB entry 6ejl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Petrvalska O, Lentini Santo D, Obsilova V, Obsil T

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158984 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Mitogen-activated protein kinase kinase kinase 5 Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 amino acids
Theoretical weight: 949 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q99683 (Residues: 963-970; Coverage: 1%)
Gene names: ASK1, MAP3K5, MAPKKK5, MEKK5

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: Excillum MetalJet D2+ 70 kV
Spacegroup: C2
Unit cell:
a: 74.98Å b: 93.427Å c: 68.491Å
α: 90° β: 99.74° γ: 90°
R-values:
R R work R free
0.197 0.196 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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