› 6emq

Solution NMR

Solution structure of the LEDGF/p75 IBD - MLL1 (aa 111-160) complex

Released:
DOI: 10.2210/pdb6emq/pdb
PDB entry 6emq coloured by chain, ensemble of 40 models, front view.
PDB entry 6emq coloured by chain, ensemble of 40 models, side view.
PDB entry 6emq coloured by chain, ensemble of 40 models, top view.
Source organism: Homo sapiens
Primary publication:
Affinity switching of the LEDGF/p75 IBD interactome is governed by kinase-dependent phosphorylation.
Sharma S, Čermáková K, De Rijck J, Demeulemeester J, Fábry M, El Ashkar S, Van Belle S, Lepšík M, Tesina P, Duchoslav V, Novák P, Hubálek M, Srb P, Christ F, Řezáčová P, Hodges HC, Debyser Z, Veverka V
Proc Natl Acad Sci U S A 115 E7053-E7062 (2018)
PMID: 29997176

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MLL cleavage product N320; PC4 and SFRS1-interacting protein Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 17.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75475 (Residues: 345-443; Coverage: 19%)
  • Canonical: Q03164 (Residues: 111-160; Coverage: 1%)
Gene names: ALL1, CXXC7, DFS70, HRX, HTRX, KMT2A, LEDGF, MLL, MLL1, PSIP1, PSIP2, TRX1
Sequence domains: Lens epithelium-derived growth factor (LEDGF)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Anti-DFS70 antibodies: an update on our current understanding and their clinical usefulness.
Mahler et al. (2019)
6 more

3 mentions without citation

Structure, function and inhibition of critical protein-protein interactions involving mixed lineage leukemia 1 and its fusion oncoproteins.
Li et al. (2021)
2 more