› 6eop

X-ray diffraction
2.4Å resolution

DPP8 - SLRFLYEG, space group 20

Released:
DOI: 10.2210/pdb6eop/pdb
PDB entry 6eop coloured by chain, front view.
PDB entry 6eop coloured by chain, side view.
PDB entry 6eop coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures and mechanism of dipeptidyl peptidases 8 and 9, important players in cellular homeostasis and cancer.
Ross B, Krapp S, Augustin M, Kierfersauer R, Arciniega M, Geiss-Friedlander R, Huber R
Proc Natl Acad Sci U S A 115 E1437-E1445 (2018)
PMID: 29382749

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159023 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dipeptidyl peptidase 8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 898 amino acids
Theoretical weight: 103.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6V1X1 (Residues: 1-898; Coverage: 100%)
Gene names: DPP8, DPRP1, MSTP097, MSTP135, MSTP141
Sequence domains:
Small ubiquitin-related modifier 1 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 8 amino acids
Theoretical weight: 985 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P63165 (Residues: 61-68; Coverage: 8%)
Gene names: OK/SW-cl.43, SMT3C, SMT3H3, SUMO1, UBL1

Ligands and Environments

3 bound ligands:
Ligand FLC 1 x FLC
Ligand CA 3 x CA
Ligand F15 3 x F15
1 modified residue:
Ligand CME 3 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 162.83Å b: 246.371Å c: 261.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.237
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

2 review citations

Structures and functions of the inflammasome engine.
Wang et al. (2021)
1 more

2 mentions without citation

Structures and mechanism of dipeptidyl peptidases 8 and 9, important players in cellular homeostasis and cancer.
Ross et al. (2018)
1 more