Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- High mobility group box domain
- High mobility group box domain superfamily
- Ribosomal RNA adenine methyltransferase KsgA/Erm
- DNA-directed RNA polymerase, phage-type
- S-adenosyl-L-methionine-dependent methyltransferase superfamily
- DNA-directed RNA polymerase, N-terminal
- Ribosomal RNA adenine methylase transferase, N-terminal
- DNA-directed RNA polymerase, C-terminal domain, phage-type
3 more domains
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119858 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Transcription factor A, mitochondrial
Molecule details ›
Chains: C, G
Length: 205 amino acids
Theoretical weight: 24.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 205 amino acids
Theoretical weight: 24.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q00059 (Residues: 43-245; Coverage: 83%)
Sequence domains:
DNA-directed RNA polymerase, mitochondrial
Molecule details ›
Chains: A, B
Length: 1128 amino acids
Theoretical weight: 127.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1128 amino acids
Theoretical weight: 127.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O00411 (Residues: 105-1230; Coverage: 92%)
Sequence domains:
Dimethyladenosine transferase 2, mitochondrial
Molecule details ›
Chains: F, J
Length: 377 amino acids
Theoretical weight: 43.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ribosomal RNA adenine dimethylase
Length: 377 amino acids
Theoretical weight: 43.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9H5Q4 (Residues: 22-396; Coverage: 95%)
Sequence domains: Ribosomal RNA adenine dimethylase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X06SA
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
