› 6ewc

X-ray diffraction
3.2Å resolution

Crystal structure of non-phosphorylated form of RLS PHOSPHOPEPTIDE BOUND TO HLA-A2 in complex with LILRB1

Released:
DOI: 10.2210/pdb6ewc/pdb
PDB entry 6ewc coloured by chain, front view.
PDB entry 6ewc coloured by chain, side view.
PDB entry 6ewc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Application of the immunoregulatory receptor LILRB1 as a crystallisation chaperone for human class I MHC complexes.
Mohammed F, Stones DH, Willcox BE
J Immunol Methods 464 47-56 (2019)
PMID: 30365927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-137821 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F
Molecule details ›
Chains: B, F
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Reticulophagy regulator 2 Chains: C, G
Molecule details ›
Chains: C, G
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8NC44 (Residues: 400-408; Coverage: 2%)
Gene names: C2orf17, FAM134A, MAG2, RETREG2
Leukocyte immunoglobulin-like receptor subfamily B member 1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 195 amino acids
Theoretical weight: 21.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NHL6 (Residues: 27-221; Coverage: 31%)
Gene names: ILT2, LILRB1, LIR1, MIR7
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 117.5Å b: 117.5Å c: 203.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Leukocyte Immunoglobulin-Like Receptors in Regulating the Immune Response in Infectious Diseases: A Window of Opportunity to Pathogen Persistence and a Sound Target in Therapeutics.
Abdallah et al. (2021)