› 6f1h

X-ray diffraction
4.5Å resolution

C1rC1s complex

Released:
DOI: 10.2210/pdb6f1h/pdb
PDB entry 6f1h coloured by chain, front view.
PDB entry 6f1h coloured by chain, side view.
PDB entry 6f1h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the C1r-C1s interaction of the C1 complex of complement activation.
Almitairi JOM, Venkatraman Girija U, Furze CM, Simpson-Gray X, Badakshi F, Marshall JE, Schwaeble WJ, Mitchell DA, Moody PCE, Wallis R
Proc Natl Acad Sci U S A 115 768-773 (2018)
PMID: 29311313

Function and Biology Details

Reactions catalysed: 
Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form C1s (EC 3.4.21.42).
Cleavage of Arg-|-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg)-|-Lys bond in complement component C2 to form C2a and C2b: the 'classical' pathway C3 convertase.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133183 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Complement C1r subcomponent heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 291 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00736 (Residues: 18-308; Coverage: 42%)
Gene name: C1R
Sequence domains:
Complement C1s subcomponent heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 276 amino acids
Theoretical weight: 31.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P09871 (Residues: 17-292; Coverage: 41%)
Gene name: C1S
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand CA 12 x CA
Ligand NA 6 x NA
Ligand NAG 1 x NAG
Ligand LYS 1 x LYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 88.209Å b: 88.34Å c: 197.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.305 0.304 0.34
Expression system: Cricetulus griseus

Quick links

Citations

8 review citations

Complement in neurological disorders and emerging complement-targeted therapeutics.
Dalakas et al. (2020)
7 more

2 mentions without citation

Structure of the C1r-C1s interaction of the C1 complex of complement activation.
Almitairi et al. (2018)
1 more