› 6f39

X-ray diffraction
5.8Å resolution

C1r homodimer CUB1-EGF-CUB2

Released:
DOI: 10.2210/pdb6f39/pdb
PDB entry 6f39 coloured by chain, front view.
PDB entry 6f39 coloured by chain, side view.
PDB entry 6f39 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the C1r-C1s interaction of the C1 complex of complement activation.
Almitairi JOM, Venkatraman Girija U, Furze CM, Simpson-Gray X, Badakshi F, Marshall JE, Schwaeble WJ, Mitchell DA, Moody PCE, Wallis R
Proc Natl Acad Sci U S A 115 768-773 (2018)
PMID: 29311313

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form C1s (EC 3.4.21.42).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Complement C1r subcomponent heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.47 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00736 (Residues: 22-306; Coverage: 41%)
Gene name: C1R
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
3 bound ligands:
Ligand CA 6 x CA
Ligand NA 2 x NA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 140.563Å b: 54.62Å c: 138.18Å
α: 90° β: 99.9° γ: 90°
R-values:
R R work R free
0.31 0.308 0.338
Expression system: Cricetulus griseus

Quick links

Citations

8 review citations

Surface loops of trypsin-like serine proteases as determinants of function.
Goettig et al. (2019)
7 more

3 mentions without citation

Structure of the C1r-C1s interaction of the C1 complex of complement activation.
Almitairi et al. (2018)
2 more