› 6f3f

X-ray diffraction
2.42Å resolution

Autoinhibited Src kinase bound to ADP

Released:
DOI: 10.2210/pdb6f3f/pdb
PDB entry 6f3f coloured by chain, front view.
PDB entry 6f3f coloured by chain, side view.
PDB entry 6f3f coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A switch in nucleotide affinity governs activation of the Src and Tec family kinases.
von Raußendorf F, de Ruiter A, Leonard TA
Sci Rep 7 17405 (2017)
PMID: 29234112

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 52.04 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05480 (Residues: 85-535; Coverage: 84%)
Gene name: Src
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 51.011Å b: 82.974Å c: 105.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.268
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Dynamic regulatory features of the protein tyrosine kinases.
Amatya et al. (2019)
3 more

3 mentions without citation

Engineering proteins for allosteric control by light or ligands.
Dagliyan et al. (2019)
2 more