› 6fbb

X-ray diffraction
1.3Å resolution

Crystal structure of 14-3-3 sigma in complex with wild-type Shroom3

Released:
DOI: 10.2210/pdb6fbb/pdb
PDB entry 6fbb coloured by chain, front view.
PDB entry 6fbb coloured by chain, side view.
PDB entry 6fbb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization of Coding/Noncoding Variants for SHROOM3 in Patients with CKD.
Prokop JW, Yeo NC, Ottmann C, Chhetri SB, Florus KL, Ross EJ, Sosonkina N, Link BA, Freedman BI, Coppola CJ, McDermott-Roe C, Leysen S, Milroy LG, Meijer FA, Geurts AM, Rauscher FJ, Ramaker R, Flister MJ, Jacob HJ, Mendenhall EM, Lazar J
J Am Soc Nephrol 29 1525-1535 (2018)
PMID: 29476007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152164 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Protein Shroom3 Chain: P
Molecule details ›
Chain: P
Length: 5 amino acids
Theoretical weight: 614 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8TF72 (Residues: 1240-1244; Coverage: 0%)
Gene names: KIAA1481, MSTP013, SHRML, SHROOM3

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand CL 2 x CL
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 82.528Å b: 112.241Å c: 62.725Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.166
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Genetic susceptibility of hypertension-induced kidney disease.
Zhang et al. (2021)
5 more

1 mention without citation

Characterization of Coding/Noncoding Variants for SHROOM3 in Patients with CKD.
Prokop et al. (2018)