› 6ff3

X-ray diffraction
2.57Å resolution

Crystal structure of Drosophila neural ectodermal development factor Imp-L1 with Human IGF-I

Released:
DOI: 10.2210/pdb6ff3/pdb
PDB entry 6ff3 coloured by chain, front view.
PDB entry 6ff3 coloured by chain, side view.
PDB entry 6ff3 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.
Roed NK, Viola CM, Kristensen O, Schluckebier G, Norrman M, Sajid W, Wade JD, Andersen AS, Kristensen C, Ganderton TR, Turkenburg JP, De Meyts P, Brzozowski AM
Nat Commun 9 3860 (2018)
PMID: 30242155

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138318 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neural/ectodermal development factor IMP-L2 Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.29 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q09024 (Residues: 26-267; Coverage: 100%)
Gene names: CG15009, ImpL2
Sequence domains: Immunoglobulin I-set domain
Insulin-like growth factor I Chain: B
Molecule details ›
Chain: B
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05019 (Residues: 49-118; Coverage: 40%)
Gene names: IBP1, IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 82.03Å b: 94.15Å c: 48.36Å
α: 90° β: 95.26° γ: 90°
R-values:
R R work R free
0.232 0.229 0.285
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

4 review citations

Insulin-like growth factors: Ligands, binding proteins, and receptors.
LeRoith et al. (2021)
3 more

1 mention without citation

Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2.
Pompach et al. (2019)