› 6fkb

X-ray diffraction
3.03Å resolution

Crystal structure of N2C/D282C stabilized opsin bound to RS13

Released:
DOI: 10.2210/pdb6fkb/pdb
PDB entry 6fkb coloured by chain, front view.
PDB entry 6fkb coloured by chain, side view.
PDB entry 6fkb coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Ligand channel in pharmacologically stabilized rhodopsin.
Mattle D, Kuhn B, Aebi J, Bedoucha M, Kekilli D, Grozinger N, Alker A, Rudolph MG, Schmid G, Schertler GFX, Hennig M, Standfuss J, Dawson RJP
Proc Natl Acad Sci U S A 115 3640-3645 (2018)
PMID: 29555765

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 37.06 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: P02699 (Residues: 1-328; Coverage: 94%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

6 bound ligands:
Ligand PLM 1 x PLM
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
Ligand MAN 2 x MAN
Ligand BOG 5 x BOG
Ligand DLH 1 x DLH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: R32
Unit cell:
a: 242.852Å b: 242.852Å c: 111.469Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.258 0.272
Expression system: Homo sapiens

Quick links

Citations

6 review citations

The Retinoid and Non-Retinoid Ligands of the Rod Visual G Protein-Coupled Receptor.
Ortega et al. (2019)
5 more

2 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
1 more