› 6flm

X-ray diffraction
2.01Å resolution

Crystal structure of the human TRIM25 PRYSPRY domain

Released:
DOI: 10.2210/pdb6flm/pdb
PDB entry 6flm coloured by chain, front view.
PDB entry 6flm coloured by chain, side view.
PDB entry 6flm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition.
Koliopoulos MG, Lethier M, van der Veen AG, Haubrich K, Hennig J, Kowalinski E, Stevens RV, Martin SR, Reis e Sousa C, Cusack S, Rittinger K
Nat Commun 9 1820 (2018)
PMID: 29739942

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin/ISG15 ligase TRIM25 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 199 amino acids
Theoretical weight: 22.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14258 (Residues: 435-630; Coverage: 31%)
Gene names: EFP, RNF147, TRIM25, ZNF147
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P321
Unit cell:
a: 152.75Å b: 152.75Å c: 68.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.206
Expression system: Trichoplusia ni

Quick links

Citations

33 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
32 more

1 mention without citation

Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1.
Muñoz Sosa et al. (2021)