› 6fo2

Electron Microscopy
4.4Å resolution

CryoEM structure of bovine cytochrome bc1 with no ligand bound

Released:
DOI: 10.2210/pdb6fo2/pdb
PDB entry 6fo2 coloured by chain, front view.
PDB entry 6fo2 coloured by chain, side view.
PDB entry 6fo2 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
X-ray and cryo-EM structures of inhibitor-bound cytochrome bc1 complexes for structure-based drug discovery.
Amporndanai K, Johnson RM, O'Neill PM, Fishwick CWG, Jamson AH, Rawson S, Muench SP, Hasnain SS, Antonyuk SV
IUCrJ 5 200-210 (2018)
PMID: 29765610
Related structures: EMD-4288

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-132265 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: A, N
Molecule details ›
Chains: A, N
Length: 480 amino acids
Theoretical weight: 52.8 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P31800 (Residues: 1-480; Coverage: 100%)
Gene name: UQCRC1
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, O
Molecule details ›
Chains: B, O
Length: 453 amino acids
Theoretical weight: 48.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P23004 (Residues: 1-453; Coverage: 100%)
Gene name: UQCRC2
Sequence domains:
Cytochrome b Chains: C, P
Molecule details ›
Chains: C, P
Length: 379 amino acids
Theoretical weight: 42.62 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00157 (Residues: 1-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome c1, heme protein, mitochondrial Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 325 amino acids
Theoretical weight: 35.34 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00125 (Residues: 1-325; Coverage: 100%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: E, R
Molecule details ›
Chains: E, R
Length: 274 amino acids
Theoretical weight: 29.59 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 1-274; Coverage: 100%)
Gene name: UQCRFS1
Sequence domains:
Cytochrome b-c1 complex subunit 7 Chains: F, S
Molecule details ›
Chains: F, S
Length: 111 amino acids
Theoretical weight: 13.5 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00129 (Residues: 1-111; Coverage: 100%)
Gene name: UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 8 Chains: G, T
Molecule details ›
Chains: G, T
Length: 82 amino acids
Theoretical weight: 9.74 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13271 (Residues: 1-82; Coverage: 100%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, U
Molecule details ›
Chains: H, U
Length: 91 amino acids
Theoretical weight: 10.64 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00126 (Residues: 1-91; Coverage: 100%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit 9 Chains: I, V
Molecule details ›
Chains: I, V
Length: 16 amino acids
Theoretical weight: 1.65 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 62-77; Coverage: 6%)
Gene name: UQCRFS1
Cytochrome b-c1 complex subunit 9 Chains: J, W
Molecule details ›
Chains: J, W
Length: 64 amino acids
Theoretical weight: 7.47 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00130 (Residues: 1-64; Coverage: 100%)
Gene name: UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand HEC 2 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-4288

Quick links

Citations

4 review citations

Tuberculosis: Past, present and future of the treatment and drug discovery research.
Bendre et al. (2021)
3 more

1 mention without citation

X-ray and cryo-EM structures of inhibitor-bound cytochrome bc1 complexes for structure-based drug discovery.
Amporndanai et al. (2018)