› 6fvh

X-ray diffraction
1.4Å resolution

Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound PITC

Released:
DOI: 10.2210/pdb6fvh/pdb
PDB entry 6fvh coloured by chain, front view.
PDB entry 6fvh coloured by chain, side view.
PDB entry 6fvh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Study of the Complex Formed by Ceruloplasmin and Macrophage Migration Inhibitory Factor.
Sokolov AV, Dadinova LA, Petoukhov MV, Bourenkov G, Dubova KM, Amarantov SV, Volkov VV, Kostevich VA, Gorbunov NP, Grudinina NA, Vasilyev VB, Samygina VR
Biochemistry (Mosc) 83 701-707 (2018)
PMID: 30195326

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand 0FI 3 x 0FI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 67.822Å b: 68.375Å c: 88.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.138 0.166
Expression system: Escherichia coli

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