› 6fxn

X-ray diffraction
2.9Å resolution

Crystal structure of human BAFF in complex with Fab fragment of anti-BAFF antibody belimumab

Released:
DOI: 10.2210/pdb6fxn/pdb
PDB entry 6fxn coloured by chain, front view.
PDB entry 6fxn coloured by chain, side view.
PDB entry 6fxn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors.
Vigolo M, Chambers MG, Willen L, Chevalley D, Maskos K, Lammens A, Tardivel A, Das D, Kowalczyk-Quintas C, Schuepbach-Mallepell S, Smulski CR, Eslami M, Rolink A, Hummler E, Samy E, Fomekong Nanfack Y, Mackay F, Liao M, Hess H, Jiang X, Schneider P
Nat Commun 9 1199 (2018)
PMID: 29572442

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212869 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tumor necrosis factor ligand superfamily member 13b, soluble form Chains: A, B, C, J, K, L
Molecule details ›
Chains: A, B, C, J, K, L
Length: 164 amino acids
Theoretical weight: 18.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y275 (Residues: 134-285; Coverage: 53%)
Gene names: BAFF, BLYS, TALL1, TNFSF13B, TNFSF20, UNQ401/PRO738, ZTNF4
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
belimumab heavy chain Chains: D, F, H, M, O, Q
Molecule details ›
Chains: D, F, H, M, O, Q
Length: 225 amino acids
Theoretical weight: 23.7 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Structure domains: Immunoglobulins
belimumab light chain Chains: E, G, I, N, P, R
Molecule details ›
Chains: E, G, I, N, P, R
Length: 214 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 135.687Å b: 135.499Å c: 138.18Å
α: 90° β: 91.88° γ: 90°
R-values:
R R work R free
0.191 0.191 0.231
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

4 review citations

BAFF and BAFF-Receptor in B Cell Selection and Survival.
Smulski et al. (2018)
3 more

1 mention without citation

Bdellovibrio bacteriovorus uses chimeric fibre proteins to recognize and invade a broad range of bacterial hosts.
Caulton et al. (2024)