› 6gdg

Electron Microscopy
4.11Å resolution

Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer

Released:
DOI: 10.2210/pdb6gdg/pdb
PDB entry 6gdg coloured by chain, front view.
PDB entry 6gdg coloured by chain, side view.
PDB entry 6gdg coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein.
García-Nafría J, Lee Y, Bai X, Carpenter B, Tate CG
Elife 7 (2018)
PMID: 29726815
Related structures: EMD-4390

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209521 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Adenosine receptor A2a; Thioredoxin 1 Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 52.91 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
  • Canonical: P29274 (Residues: 8-316; Coverage: 75%)
Gene names: ADORA2, ADORA2A, JW5856, b3781, fipA, trxA, tsnC
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: D
Molecule details ›
Chain: D
Length: 248 amino acids
Theoretical weight: 28.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 6-64, 204-394; Coverage: 61%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
nanobody Nb35 Chain: E
Molecule details ›
Chain: E
Length: 156 amino acids
Theoretical weight: 16.93 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NEC 1 x NEC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.11Å
Relevant EMDB volumes: EMD-4390
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

26 review citations

Adenosine A2A receptor antagonists: from caffeine to selective non-xanthines.
Jacobson et al. (2022)
25 more

37 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
36 more