› 6ghd

X-ray diffraction
2.1Å resolution

Structural analysis of the ternary complex between lamin A/C, BAF and emerin identifies an interface disrupted in autosomal recessive progeroid diseases

Released:
DOI: 10.2210/pdb6ghd/pdb
PDB entry 6ghd coloured by chain, front view.
PDB entry 6ghd coloured by chain, side view.
PDB entry 6ghd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the ternary complex between lamin A/C, BAF and emerin identifies an interface disrupted in autosomal recessive progeroid diseases.
Samson C, Petitalot A, Celli F, Herrada I, Ropars V, Le Du MH, Nhiri N, Jacquet E, Arteni AA, Buendia B, Zinn-Justin S
Nucleic Acids Res 46 10460-10473 (2018)
PMID: 30137533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131038 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Emerin Chains: G, H
Molecule details ›
Chains: G, H
Length: 45 amino acids
Theoretical weight: 5.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50402 (Residues: 2-45; Coverage: 17%)
Gene names: EDMD, EMD, STA
Sequence domains: LEM domain
Structure domains: Transcription Termination Factor Rho, Rna-binding Domain; Chain A, Domain 1
Barrier-to-autointegration factor Chains: A, C, D, E
Molecule details ›
Chains: A, C, D, E
Length: 88 amino acids
Theoretical weight: 9.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75531 (Residues: 2-89; Coverage: 99%)
Gene names: BAF, BANF1, BCRG1
Sequence domains: Barrier to autointegration factor
Structure domains: Barrier-to-autointegration factor, BAF
Lamin-A/C Chains: B, F
Molecule details ›
Chains: B, F
Length: 119 amino acids
Theoretical weight: 13.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02545 (Residues: 428-546; Coverage: 18%)
Gene names: LMN1, LMNA
Sequence domains: Lamin Tail Domain
Structure domains: Lamin Tail domain

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P1
Unit cell:
a: 57.77Å b: 62.75Å c: 64.34Å
α: 66.44° β: 66.55° γ: 88.05°
R-values:
R R work R free
0.194 0.192 0.247
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Lamin A/C: Function in Normal and Tumor Cells.
Dubik et al. (2020)
8 more

6 mentions without citation

Recent insight into intermediate filament structure.
Eldirany et al. (2021)
5 more