› 6gis

X-ray diffraction
2.82Å resolution

Structural basis of human clamp sliding on DNA

Released:
DOI: 10.2210/pdb6gis/pdb
PDB entry 6gis coloured by chain, front view.
PDB entry 6gis coloured by chain, side view.
PDB entry 6gis coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of human PCNA sliding on DNA.
De March M, Merino N, Barrera-Vilarmau S, Crehuet R, Onesti S, Blanco FJ, De Biasio A
Nat Commun 8 13935 (2017)
PMID: 28071730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119955 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 262 amino acids
Theoretical weight: 28.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA (5'-D(P*AP*TP*AP*CP*GP*AP*TP*GP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.11 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*CP*CP*AP*TP*CP*GP*TP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 10 nucleotides
Theoretical weight: 2.98 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: R3
Unit cell:
a: 180.189Å b: 180.189Å c: 76.832Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.247 0.284
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation.
Esadze et al. (2018)
8 more

9 mentions without citation

Human PCNA Structure, Function and Interactions.
González-Magaña et al. (2020)
8 more