› 6gws

X-ray diffraction
2.9Å resolution

Crystal structure of human PCNA in complex with three p15 peptides

Released:
DOI: 10.2210/pdb6gws/pdb
PDB entry 6gws coloured by chain, front view.
PDB entry 6gws coloured by chain, side view.
PDB entry 6gws coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
p15PAF binding to PCNA modulates the DNA sliding surface.
De March M, Barrera-Vilarmau S, Crespan E, Mentegari E, Merino N, Gonzalez-Magaña A, Romano-Moreno M, Maga G, Crehuet R, Onesti S, Blanco FJ, De Biasio A
Nucleic Acids Res 46 9816-9828 (2018)
PMID: 30102405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146059 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 264 amino acids
Theoretical weight: 29.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
PCNA-associated factor Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 32 amino acids
Theoretical weight: 3.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15004 (Residues: 41-72; Coverage: 29%)
Gene names: KIAA0101, L5, NS5ATP9, PAF, PCLAF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 79.24Å b: 89.75Å c: 85.13Å
α: 90° β: 117.25° γ: 90°
R-values:
R R work R free
0.203 0.2 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

DNA damage tolerance in stem cells, ageing, mutagenesis, disease and cancer therapy.
Pilzecker et al. (2019)
1 more

3 mentions without citation

Human PCNA Structure, Function and Interactions.
González-Magaña et al. (2020)
2 more