› 6h7x

X-ray diffraction
2.89Å resolution

First X-ray structure of full-length human RuvB-Like 2.

Released:
DOI: 10.2210/pdb6h7x/pdb
PDB entry 6h7x coloured by chain, front view.
PDB entry 6h7x coloured by chain, side view.
PDB entry 6h7x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structure of full-length human RuvB-Like 2 - mechanistic insights into coupling between ATP binding and mechanical action.
Silva STN, Brito JA, Arranz R, Sorzano CÓS, Ebel C, Doutch J, Tully MD, Carazo JM, Carrascosa JL, Matias PM, Bandeiras TM
Sci Rep 8 13726 (2018)
PMID: 30213962

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RuvB-like 2 Chain: A
Molecule details ›
Chain: A
Length: 463 amino acids
Theoretical weight: 51.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y230 (Residues: 1-463; Coverage: 100%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand EDO 7 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6
Unit cell:
a: 123.023Å b: 123.023Å c: 60.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.237
Expression system: Escherichia coli

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Citations

1 review citation

RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes.
Rodríguez et al. (2020)
4 other articles

5 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
4 more