› 6mbl

X-ray diffraction
2.2Å resolution

SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, Second P212121 Crystal Form

Released:
DOI: 10.2210/pdb6mbl/pdb
PDB entry 6mbl coloured by chain, front view.
PDB entry 6mbl coloured by chain, side view.
PDB entry 6mbl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SETD3 is an actin histidine methyltransferase that prevents primary dystocia.
Wilkinson AW, Diep J, Dai S, Liu S, Ooi YS, Song D, Li TM, Horton JR, Zhang X, Liu C, Trivedi DV, Ruppel KM, Vilches-Moure JG, Casey KM, Mak J, Cowan T, Elias JE, Nagamine CM, Spudich JA, Cheng X, Carette JE, Gozani O
Nature 565 372-376 (2019)
PMID: 30626964

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein N(tau)-methyl-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1, N-terminally processed Chain: Y
Molecule details ›
Chain: Y
Length: 15 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P60709 (Residues: 66-80; Coverage: 4%)
Gene name: ACTB
Actin-histidine N-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 67.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86TU7 (Residues: 1-594; Coverage: 100%)
Gene names: C14orf154, SETD3
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 56.557Å b: 92.83Å c: 111.635Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.227
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Histone lysine methyltransferases in biology and disease.
Husmann et al. (2019)
14 more

4 mentions without citation

Enzymology and significance of protein histidine methylation.
Jakobsson ME. (2021)
3 more