› 6miw

X-ray diffraction
2Å resolution

WWE domain of human HUWE1

Released:
DOI: 10.2210/pdb6miw/pdb
PDB entry 6miw coloured by chain, front view.
PDB entry 6miw coloured by chain, side view.
PDB entry 6miw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Halabelian L, Loppnau P, Tempel W, Wong F, Bountra C, Arrowsmith CH, Edwards AM, Tong Y, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HUWE1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 10.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6Z7 (Residues: 1611-1700; Coverage: 2%)
Gene names: HSPC272, HUWE1, KIAA0312, KIAA1578, UREB1
Sequence domains: WWE domain

Ligands and Environments

2 bound ligands:
Ligand UNX 10 x UNX
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P3221
Unit cell:
a: 60.134Å b: 60.134Å c: 53.288Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.18 0.212
Expression system: Escherichia coli

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