› 6a3v

X-ray diffraction
3.39Å resolution

Complex structure of human 4-1BB and 4-1BBL

Released:
DOI: 10.2210/pdb6a3v/pdb
PDB entry 6a3v coloured by chain, front view.
PDB entry 6a3v coloured by chain, side view.
PDB entry 6a3v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Limited Cross-Linking of 4-1BB by 4-1BB Ligand and the Agonist Monoclonal Antibody Utomilumab.
Li Y, Tan S, Zhang C, Chai Y, He M, Zhang CW, Wang Q, Tong Z, Liu K, Lei Y, Liu WJ, Liu Y, Tian Z, Cao X, Yan J, Qi J, Tien P, Gao S, Gao GF
Cell Rep 25 909-920.e4 (2018)
PMID: 30355497

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154370 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor ligand superfamily member 9 Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Length: 203 amino acids
Theoretical weight: 21.52 KDa
Source organism: Homo sapiens
Expression system: Baculovirus transfer vector pFASTBAC1
UniProt:
  • Canonical: P41273 (Residues: 58-254; Coverage: 78%)
Gene name: TNFSF9
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Tumor necrosis factor receptor superfamily member 9 Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 163 amino acids
Theoretical weight: 17.52 KDa
Source organism: Homo sapiens
Expression system: Baculovirus transfer vector pFASTBAC1
UniProt:
  • Canonical: Q07011 (Residues: 24-180; Coverage: 68%)
Gene names: CD137, ILA, TNFRSF9
Sequence domains: TNFR/NGFR cysteine-rich region

Ligands and Environments

1 bound ligand:
Ligand NAG 12 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 126.091Å b: 137.195Å c: 140.318Å
α: 90° β: 111.8° γ: 90°
R-values:
R R work R free
0.265 0.264 0.293
Expression system: Baculovirus transfer vector pFASTBAC1

Quick links

Citations

10 review citations

Development of bispecific antibodies in China: overview and prospects.
Zhang et al. (2020)
9 more

3 mentions without citation

Generation of a safe and efficacious llama single-domain antibody fragment (vHH) targeting the membrane-proximal region of 4-1BB for engineering therapeutic bispecific antibodies for cancer.
Zhai et al. (2021)
2 more