› 6a8r

X-ray diffraction
1.6Å resolution

Crystal structure of DUX4 HD2 domain associated with ERG DNA binding site

Released:
DOI: 10.2210/pdb6a8r/pdb
PDB entry 6a8r coloured by chain, front view.
PDB entry 6a8r coloured by chain, side view.
PDB entry 6a8r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DUX4HD2-DNAERG structure reveals new insight into DUX4-Responsive-Element.
Dong X, Zhang H, Cheng N, Li K, Meng G
Leukemia 33 550-553 (2019)
PMID: 30315230

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119550 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Double homeobox protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBX2 (Residues: 94-153; Coverage: 14%)
Gene names: DUX10, DUX4
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(P*TP*GP*AP*TP*GP*AP*GP*AP*TP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*AP*AP*TP*CP*TP*CP*AP*TP*CP*A)-3') Chain: F
Molecule details ›
Chain: F
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P31
Unit cell:
a: 32.55Å b: 32.55Å c: 126.591Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.2 0.207
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

DUX Hunting-Clinical Features and Diagnostic Challenges Associated with DUX4-Rearranged Leukaemia.
Rehn et al. (2020)
1 more

1 mention without citation

DNA crosslinking and recombination-activating genes 1/2 (RAG1/2) are required for oncogenic splicing in acute lymphoblastic leukemia.
Zhang et al. (2021)