› 6ac6

X-ray diffraction
2.99Å resolution

Ab initio crystal structure of Selenomethionine labelled Mycobacterium smegmatis Mfd

Released:
DOI: 10.2210/pdb6ac6/pdb
PDB entry 6ac6 coloured by chain, front view.
PDB entry 6ac6 coloured by chain, side view.
PDB entry 6ac6 coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Entry authors: Putta S, Fox GC, Walsh MA, Rao DN, Nagaraja V, Natesh R

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription-repair-coupling factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 1235 amino acids
Theoretical weight: 134.7 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0R3C5 (Residues: 1-1215; Coverage: 100%)
Gene names: MSMEG_5423, mfd
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 54 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 82.71Å b: 158.87Å c: 207.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.27
Expression system: Escherichia coli

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