› 6ak3

X-ray diffraction
2.9Å resolution

Crystal structure of the human prostaglandin E receptor EP3 bound to prostaglandin E2

Released:
DOI: 10.2210/pdb6ak3/pdb
PDB entry 6ak3 coloured by chain, front view.
PDB entry 6ak3 coloured by chain, side view.
PDB entry 6ak3 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the endogenous agonist-bound prostanoid receptor EP3.
Morimoto K, Suno R, Hotta Y, Yamashita K, Hirata K, Yamamoto M, Narumiya S, Iwata S, Kobayashi T
Nat Chem Biol 15 8-10 (2019)
PMID: 30510192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prostaglandin E2 receptor EP3 subtype; Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 46.04 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43115 (Residues: 43-359; Coverage: 81%)
  • Canonical: P0ABE7 (Residues: 23-62, 85-127; Coverage: 78%)
Gene names: PTGER3, cybC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand P2E 2 x P2E
Ligand POV 2 x POV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 66.09Å b: 42.28Å c: 161.3Å
α: 90° β: 96.09° γ: 90°
R-values:
R R work R free
0.253 0.251 0.295
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Roles of polyunsaturated fatty acids, from mediators to membranes.
Harayama et al. (2020)
1 more

19 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
18 more