› 6amk

X-ray diffraction
3.29Å resolution

Structure of Streptomyces venezuelae BldC-whiI opt complex

Released:
DOI: 10.2210/pdb6amk/pdb
PDB entry 6amk coloured by chain, front view.
PDB entry 6amk coloured by chain, side view.
PDB entry 6amk coloured by chain, top view.
Source organism: Streptomyces venezuelae
Primary publication:
The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.
Schumacher MA, den Hengst CD, Bush MJ, Le TBK, Tran NT, Chandra G, Zeng W, Travis B, Brennan RG, Buttner MJ
Nat Commun 9 1139 (2018)
PMID: 29556010

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119568 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH merR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.16 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli
UniProt:
  • Canonical: F2REK9 (Residues: 2-9, 10-68; Coverage: 99%)
Gene name: SVEN_3846
Sequence domains: Helix-turn-helix domain
DNA (5'-D(*TP*TP*CP*AP*AP*TP*TP*CP*GP*GP*GP*TP*AP*AP*TP*TP*CP*GP*GP*GP*CP*A)-3') Chain: R
Molecule details ›
Chain: R
Length: 22 nucleotides
Theoretical weight: 6.78 KDa
DNA (5'-D(*AP*AP*TP*GP*TP*CP*CP*GP*AP*AP*TP*TP*AP*CP*CP*CP*GP*AP*AP*TP*TP*G)-3') Chain: Z
Molecule details ›
Chain: Z
Length: 22 nucleotides
Theoretical weight: 6.74 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6122
Unit cell:
a: 114.2Å b: 114.2Å c: 159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.223 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

When is a transcription factor a NAP?
Dorman et al. (2020)
16 other articles

1 mention without citation

The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.
Schumacher et al. (2018)