› 6anh

X-ray diffraction
2.65Å resolution

Crystal structure of PPK2 class III in complex with Guanosine 5-tetraphosphate

Released:
DOI: 10.2210/pdb6anh/pdb
PDB entry 6anh coloured by chain, front view.
PDB entry 6anh coloured by chain, side view.
PDB entry 6anh coloured by chain, top view.
Source organism: Cytophaga hutchinsonii ATCC 33406
Primary publication:
Structural Insights into Substrate Selectivity and Activity of Bacterial Polyphosphate Kinases
Nocek B, Khusnutdinova AN, Ruszkowski M, Flick R, Burda M, Batyrova K, Brown G, Mucha A, Joachimiak A, Berlicki L, Yakunin AF
ACS Catalysis 8 10746-10760 (2018)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphosphate:AMP phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.54 KDa
Source organism: Cytophaga hutchinsonii ATCC 33406
Expression system: Escherichia coli
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand BKP 1 x BKP
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4122
Unit cell:
a: 110.812Å b: 110.812Å c: 178.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.238
Expression system: Escherichia coli

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