6anu

Electron Microscopy
7Å resolution

Cryo-EM structure of F-actin complexed with the beta-III-spectrin actin-binding domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-127265 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60709 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Spectrin beta chain, non-erythrocytic 2 Chains: a, b, c, d, e, f
Molecule details ›
Chains: a, b, c, d, e, f
Length: 284 amino acids
Theoretical weight: 32.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15020 (Residues: 1-284; Coverage: 12%)
Gene names: KIAA0302, SCA5, SPTBN2
Sequence domains: Calponin homology (CH) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-8886
Expression system: Escherichia coli