› 6anv

X-ray diffraction
2.26Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 49.77 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7P7M1 (Residues: 1-78; Coverage: 100%)
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JBD30_035, JW3994, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand MES 2 x MES
Ligand PGE 3 x PGE
Ligand EDO 7 x EDO
Ligand PEG 7 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 87.389Å b: 63.245Å c: 96.733Å
α: 90° β: 93.21° γ: 90°
R-values:
R R work R free
0.168 0.165 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
25 more

4 mentions without citation

Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs.
Trasanidou et al. (2019)
3 more