6az1

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the small subunit of Leishmania ribosome bound to paromomycin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 38-mer (preferred)
PDBe Complex ID:
PDB-CPX-104709 (preferred)
Entry contents:
33 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (38 distinct):
Small ribosomal subunit protein eS1 Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 30.09 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BRS2 (Residues: 1-264; Coverage: 100%)
Gene names: CGC20_0740, CGC20_0745, CGC21_5810, CGC21_5815, LDBPK_350400, LDBPK_350410, LDHU3_35.0560, LDHU3_35.0570, LdCL_350009100, LdCL_350009200
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 27.56 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A4IDS4 (Residues: 1-246; Coverage: 100%)
Gene names: LinJ36.5570, LinJ36.5680, LinJ_36_5240, LinJ_36_5350
Sequence domains: Ribosomal protein S2
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1
40S ribosomal protein S3 Chain: C
Molecule details ›
Chain: C
Length: 219 amino acids
Theoretical weight: 24.51 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BQ25 (Residues: 124-342; Coverage: 64%)
Gene name: LDBPK_330960
Sequence domains:
Structure domains:
40S ribosomal protein S9, putative Chain: D
Molecule details ›
Chain: D
Length: 190 amino acids
Theoretical weight: 22.18 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S5H5Z2 (Residues: 1-190; Coverage: 100%)
Gene names: CGC20_1155, CGC20_29260, CGC21_12965, LDBPK_361310, LDHU3_07.0910, LDHU3_36.1650, LdCL_070012400, LdCL_360018200
Sequence domains:
40S ribosomal protein S4 Chain: E
Molecule details ›
Chain: E
Length: 273 amino acids
Theoretical weight: 30.71 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3Q8I8K3 (Residues: 1-273; Coverage: 100%)
Gene names: LDBPK_131120, LDHU3_13.1380, LDHU3_13.1390, LdCL_130016500, LdCL_130016600
Sequence domains:
Structure domains:
S5 DRBM domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 265 amino acids
Theoretical weight: 28.8 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S7X558 (Residues: 1-7, 8-264; Coverage: 100%)
Gene names: LDBPK_320460, LDHU3_32.0560, LdCL_320009500
Sequence domains:
Structure domains:
Small ribosomal subunit protein eS6 Chain: G
Molecule details ›
Chain: G
Length: 249 amino acids
Theoretical weight: 28.34 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: O44012 (Residues: 1-249; Coverage: 100%)
Gene names: RPS6, RS6
Sequence domains: Ribosomal protein S6e
Small ribosomal subunit protein uS7 domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 190 amino acids
Theoretical weight: 21.31 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A381MCG8 (Residues: 1-190; Coverage: 100%)
Gene name: LINJ_11_0960
Sequence domains: Ribosomal protein S7p/S5e
Structure domains: Ribosomal protein S7 domain
40S ribosomal protein S7 Chain: I
Molecule details ›
Chain: I
Length: 200 amino acids
Theoretical weight: 23.87 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S7WNJ7 (Residues: 1-200; Coverage: 100%)
Gene names: CGC20_4385, CGC20_4390, CGC21_17890, CGC21_17895, LDBPK_010440, LDHU3_01.0460, LDHU3_01.0470, LdCL_010009300, LdCL_010009400
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S15A Chain: J
Molecule details ›
Chain: J
Length: 130 amino acids
Theoretical weight: 14.72 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BAV6 (Residues: 1-130; Coverage: 100%)
Gene names: CGC20_25145, CGC20_31130, CGC21_28485, LDBPK_111180, LDBPK_291920, LDHU3_11.1430, LDHU3_29.2740, LdCL_110017700, LdCL_290024900
Sequence domains: Ribosomal protein S8
Structure domains:
40S ribosomal protein S8 Chain: K
Molecule details ›
Chain: K
Length: 220 amino acids
Theoretical weight: 25.04 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3Q8ID68 (Residues: 1-220; Coverage: 100%)
Gene names: CGC21_16660, CGC21_16665, LDBPK_242160, LdCL_240027200, LdCL_240027300
Sequence domains: Ribosomal protein S8e
40S ribosomal protein S16 Chain: L
Molecule details ›
Chain: L
Length: 149 amino acids
Theoretical weight: 16.72 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BI96 (Residues: 1-149; Coverage: 100%)
Gene names: CGC20_4945, CGC21_31780, CGC21_31785, LDBPK_260840, LDBPK_260850, LDHU3_26.1100, LdCL_260014100, LdCL_260014200
Sequence domains: Ribosomal protein S9/S16
Structure domains: Ribosomal Protein S5; domain 2
Small ribosomal subunit protein uS10 domain-containing protein Chain: M
Molecule details ›
Chain: M
Length: 116 amino acids
Theoretical weight: 13.05 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BJI2 (Residues: 1-116; Coverage: 100%)
Gene names: CGC20_13545, CGC20_13555, CGC21_22050, LDBPK_281100, LDBPK_281120, LDHU3_28.1350, LDHU3_28.1370, LdCL_280015600, LdCL_280015800
Sequence domains: Ribosomal protein S10p/S20e
Structure domains: Ribosomal protein S10 domain
Plectin/eS10 N-terminal domain-containing protein Chain: N
Molecule details ›
Chain: N
Length: 153 amino acids
Theoretical weight: 17.43 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BTG4 (Residues: 1-153; Coverage: 100%)
Gene name: LDBPK_361040
Sequence domains: Plectin/S10 domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
40S ribosomal protein S14 Chain: O
Molecule details ›
Chain: O
Length: 144 amino acids
Theoretical weight: 15.61 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S7X3Z7 (Residues: 1-144; Coverage: 100%)
Gene names: LDBPK_303650, LDHU3_30.4850, LdCL_300041900
Sequence domains: Ribosomal protein S11
Structure domains: Ribosomal protein S11
40S ribosomal protein S23 Chain: P
Molecule details ›
Chain: P
Length: 143 amino acids
Theoretical weight: 15.95 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BFB6 (Residues: 1-143; Coverage: 100%)
Gene names: CGC20_15985, CGC20_22285, CGC20_22290, CGC21_34300, CGC21_34305, LDBPK_211300, LDBPK_211310, LDHU3_21.1530, LDHU3_21.1540, LdCL_210018100, LdCL_210018200
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S12 Chain: Q
Molecule details ›
Chain: Q
Length: 141 amino acids
Theoretical weight: 15.61 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S5H6N5 (Residues: 1-141; Coverage: 100%)
Gene names: CGC20_17345, CGC21_33180, LDBPK_130460, LDHU3_13.0630, LdCL_130010000
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
40S ribosomal protein S18 Chain: R
Molecule details ›
Chain: R
Length: 153 amino acids
Theoretical weight: 17.44 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BTF9 (Residues: 14-166; Coverage: 92%)
Gene names: LDBPK_360990, LDBPK_361000, LdCL_360015000
Sequence domains: Ribosomal protein S13/S18
Ribosomal protein S29 Chain: S
Molecule details ›
Chain: S
Length: 57 amino acids
Theoretical weight: 6.7 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BJV7 (Residues: 1-57; Coverage: 100%)
Gene names: LDBPK_282360, LDBPK_282650, LDHU3_28.3200, LDHU3_28.3580, LdCL_280028700, LdCL_280031600
Sequence domains: Ribosomal protein S14p/S29e
Structure domains: 30s Ribosomal Protein S14; Chain N
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: T
Molecule details ›
Chain: T
Length: 151 amino acids
Theoretical weight: 17.46 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S7X790 (Residues: 1-151; Coverage: 100%)
Gene names: CGC20_9130, CGC20_9780, CGC21_5575, LDBPK_333300, LDHU3_19.0440, LDHU3_33.4780, LdCL_330040600
Sequence domains:
Structure domains: S15/NS1, RNA-binding
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: U
Molecule details ›
Chain: U
Length: 173 amino acids
Theoretical weight: 19.99 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: Q9TXA6 (Residues: 1-141; Coverage: 100%)
Gene names: LDHU3_20.1990, LDHU3_21.2120, LdCL_200021000, LdCL_210023700
Sequence domains:
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
40S ribosomal protein S17 Chain: V
Molecule details ›
Chain: V
Length: 143 amino acids
Theoretical weight: 16.52 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BJZ5 (Residues: 1-143; Coverage: 100%)
Gene names: CGC20_12800, CGC20_12805, CGC21_22780, LDBPK_282750, LDBPK_282760, LDHU3_28.3730, LDHU3_28.3740, LdCL_280032600, LdCL_280032700
Sequence domains: Ribosomal S17
Structure domains: Ribosomal protein S17e-like
40S ribosomal protein S15 Chain: W
Molecule details ›
Chain: W
Length: 152 amino acids
Theoretical weight: 17.53 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BFQ0 (Residues: 1-101; Coverage: 100%)
Gene name: LDBPK_220310
Sequence domains: Ribosomal protein S19
40S ribosomal protein S19 protein Chain: X
Molecule details ›
Chain: X
Length: 179 amino acids
Theoretical weight: 20.19 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BR60 (Residues: 1-179; Coverage: 100%)
Gene name: LDBPK_342620
Sequence domains: Ribosomal protein S19e
40S ribosomal protein S21 Chain: Y
Molecule details ›
Chain: Y
Length: 159 amino acids
Theoretical weight: 16.67 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BAR6 (Residues: 122-202; Coverage: 40%)
Gene name: LDBPK_110780
Sequence domains: Ribosomal protein S21e
Structure domains: Hypothetical Cytosolic Protein; Chain: A;
40S ribosomal protein S24 Chain: Z
Molecule details ›
Chain: Z
Length: 137 amino acids
Theoretical weight: 15.81 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3Q8IKW0 (Residues: 1-137; Coverage: 100%)
Gene names: CGC20_28470, CGC21_13790, CGC21_13795, CGC21_13800, LDBPK_363020, LDHU3_36.4170, LDHU3_36.4180, LdCL_360036500, LdCL_360036600
Sequence domains: Ribosomal protein S24e
Structure domains: Alpha-Beta Plaits
40S ribosomal protein S25 Chain: a
Molecule details ›
Chain: a
Length: 120 amino acids
Theoretical weight: 13.07 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BHM6 (Residues: 1-120; Coverage: 100%)
Gene names: CGC20_2335, CGC20_26645, CGC21_25565, CGC21_8675, LDBPK_251220, LDBPK_340460, LDHU3_25.1560, LDHU3_34.0630, LdCL_250018000, LdCL_340009700
Sequence domains: S25 ribosomal protein
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
40S ribosomal protein S26 Chain: b
Molecule details ›
Chain: b
Length: 112 amino acids
Theoretical weight: 12.79 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BK95 (Residues: 1-112; Coverage: 100%)
Gene names: CGC20_13810, CGC20_6240, CGC21_36310, LDBPK_280570, LDBPK_303240, LDHU3_28.0690, LDHU3_30.4330, LdCL_280010400, LdCL_300038000
Sequence domains: Ribosomal protein S26e
Structure domains: Ribosomal protein S26e
40S_ribosomal_protein_S27-1_putative/GeneDB:LmjF. 36.3750 Chain: c
Molecule details ›
Chain: c
Length: 86 amino acids
Theoretical weight: 9.82 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3Q8ID11 (Residues: 1-86; Coverage: 100%)
Gene names: LDBPK_363940, LDHU3_18.0800, LDHU3_36.5280, LdCL_180011100, LdCL_360046400
Sequence domains: Ribosomal protein S27
Structure domains: Zn-binding ribosomal proteins
40S ribosomal protein S33 Chain: d
Molecule details ›
Chain: d
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BIH3 (Residues: 1-87; Coverage: 100%)
Gene names: LDBPK_261610, LDBPK_261620, LDHU3_26.2030, LDHU3_26.2040, LdCL_260021800, LdCL_260021900
Sequence domains: Ribosomal protein S28e
Structure domains: Nucleic acid-binding proteins
40S ribosomal protein S30 Chain: e
Molecule details ›
Chain: e
Length: 66 amino acids
Theoretical weight: 7.39 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: E9BLG3 (Residues: 1-66; Coverage: 100%)
Gene names: LDBPK_300690, LDBPK_300710, LDHU3_30.0880, LDHU3_30.0890, LDHU3_30.0900, LdCL_300012000, LdCL_300012100, LdCL_300012200
Sequence domains: Ribosomal protein S30
Ubiquitin-ribosomal protein eL40 fusion protein Chain: f
Molecule details ›
Chain: f
Length: 152 amino acids
Theoretical weight: 17.02 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: A0A3S7XAC2 (Residues: 1-152; Coverage: 100%)
Gene names: CGC21_12655, LDBPK_360660, LdCL_360011500
Sequence domains:
LACK protective antigen Chain: g
Molecule details ›
Chain: g
Length: 312 amino acids
Theoretical weight: 34.46 KDa
Source organism: Leishmania donovani
UniProt:
  • Canonical: Q9BIJ5 (Residues: 1-312; Coverage: 100%)
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
18S Ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 2203 nucleotides
Theoretical weight: 710.29 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
tRNA-Phe Chain: 2
Molecule details ›
Chain: 2
Length: 76 nucleotides
Theoretical weight: 24.6 KDa
Sequence domains: tRNA
P-site tRNA Chain: 3
Molecule details ›
Chain: 3
Length: 77 nucleotides
Theoretical weight: 24.79 KDa
Sequence domains: tRNA
E-site tRNA Chain: 4
Molecule details ›
Chain: 4
Length: 76 nucleotides
Theoretical weight: 24.49 KDa
Sequence domains: tRNA
Messenger RNA Chain: 5
Molecule details ›
Chain: 5
Length: 13 nucleotides
Theoretical weight: 4.09 KDa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-7024