6b6h

Electron Microscopy
3.9Å resolution

The cryo-EM structure of a bacterial class I transcription activation complex

Released:
Primary publication:
Structural basis of bacterial transcription activation.
Science 358 947-951 (2017)
PMID: 29146813
Related structures: EMD-7059

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-199488 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (10 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli S88
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli S88
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
RNA polymerase sigma factor RpoD Chain: F
Molecule details ›
Chain: F
Length: 628 amino acids
Theoretical weight: 72.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00579 (Residues: 1-613; Coverage: 100%)
Gene names: JW3039, alt, b3067, rpoD
Sequence domains:
DNA-binding transcriptional dual regulator CRP Chains: G, H
Molecule details ›
Chains: G, H
Length: 210 amino acids
Theoretical weight: 23.67 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit alpha Chain: I
Molecule details ›
Chain: I
Length: 75 amino acids
Theoretical weight: 8.35 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 250-324; Coverage: 23%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain
SYNTHETIC NONTEMPLATE STRAND DNA (88-MER) Chain: 1
Molecule details ›
Chain: 1
Length: 88 nucleotides
Theoretical weight: 27.13 KDa
SYNTHETIC TEMPLATE STRAND DNA (88-MER) Chain: 2
Molecule details ›
Chain: 2
Length: 88 nucleotides
Theoretical weight: 27.22 KDa
NASCENT RNA 3-MER Chain: 3
Molecule details ›
Chain: 3
Length: 3 nucleotides
Theoretical weight: 1.13 KDa

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-7059
Expression system: Escherichia coli