Function and Biology Details
Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Ras-related protein Rap-1b and Talin-1 (preferred)
PDBe Complex ID:
PDB-CPX-150643 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Talin-1
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: N-terminal or F0 domain of Talin-head FERM
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P26039 (Residues: 1-86; Coverage: 3%)
Sequence domains: N-terminal or F0 domain of Talin-head FERM
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ras-related protein Rap-1b
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 19.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Length: 168 amino acids
Theoretical weight: 19.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P61224 (Residues: 1-167; Coverage: 91%)
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
74%
Refinement method:
simulated annealing
Expression system: Escherichia coli BL21(DE3)
