› 6bad

X-ray diffraction
2.1Å resolution

Lactate Dehydrogenase in complex with inhibitor (R)-3-((2-chlorophenyl)thio)-6-(3-((4-fluorophenyl)amino)phenyl)-4-hydroxy-6-(thiophen-3-yl)-5,6-dihydro-2H-pyran-2-one

Released:
DOI: 10.2210/pdb6bad/pdb
PDB entry 6bad coloured by chain, front view.
PDB entry 6bad coloured by chain, side view.
PDB entry 6bad coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ultsch M, Eigenbrot C

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 36.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
3 bound ligands:
Ligand EPE 7 x EPE
Ligand SO4 5 x SO4
Ligand D0Y 4 x D0Y
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 75.401Å b: 80.714Å c: 102.429Å
α: 90° β: 96.81° γ: 90°
R-values:
R R work R free
0.194 0.194 0.23
Expression system: Escherichia coli

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