› 6bch

X-ray diffraction
3Å resolution

I-LtrI E29D bound to cognate substrate (nicked complex)

Released:
DOI: 10.2210/pdb6bch/pdb
PDB entry 6bch coloured by chain, front view.
PDB entry 6bch coloured by chain, side view.
PDB entry 6bch coloured by chain, top view.
Source organism: Leptographium truncatum
Entry authors: Brown C, Zhang K, McMurrough TA, Gloor GB, Edgell DR, Junop M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111775 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, D, H, L
Molecule details ›
Chains: A, D, H, L
Length: 315 amino acids
Theoretical weight: 36 KDa
Source organism: Leptographium truncatum
Expression system: Escherichia coli
UniProt:
  • Canonical: C7SWF3 (Residues: 398-712; Coverage: 44%)
Gene names: HEG fusion, rps3
Sequence domains: LAGLIDADG endonuclease
DNA (5'-D(*GP*GP*TP*CP*TP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*T)-3') Chains: B, E, I, N
Molecule details ›
Chains: B, E, I, N
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Leptographium truncatum
Expression system: Not provided
DNA (26-MER) Chains: C, F, J, O
Molecule details ›
Chains: C, F, J, O
Length: 26 nucleotides
Theoretical weight: 7.93 KDa
Source organism: Leptographium truncatum
Expression system: Not provided
DNA (5'-D(*TP*AP*GP*GP*AP*GP*CP*AP*TP*TP*T)-3') Chains: G, K, P, X
Molecule details ›
Chains: G, K, P, X
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Leptographium truncatum
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 43.76Å b: 66.315Å c: 169.924Å
α: 90.03° β: 90.03° γ: 90.22°
R-values:
R R work R free
0.257 0.254 0.306
Expression systems:
  • Escherichia coli
  • Not provided

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