6bcr

X-ray diffraction
1.99Å resolution

Complex of 14-3-3 theta with an IRSp53 peptide phosphorylated at T340

Released:
Source organism: Homo sapiens
Primary publication:
Mechanism of IRSp53 inhibition by 14-3-3.
Nat Commun 10 483 (2019)
PMID: 30696821

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-150979 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein theta Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 245 amino acids
Theoretical weight: 27.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27348 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAQ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
BAR/IMD domain-containing adapter protein 2 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 14 amino acids
Theoretical weight: 1.65 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQB8 (Residues: 333-346; Coverage: 3%)
Gene name: BAIAP2

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P1
Unit cell:
a: 60.007Å b: 69.114Å c: 84.56Å
α: 105.25° β: 95.72° γ: 115.04°
R-values:
R R work R free
0.196 0.195 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided