6bcu

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the activated RHEB-mTORC1 refined to 3.4 angstrom

Released:
Source organism: Homo sapiens
Related structures: EMD-7086

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154630 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 2549 amino acids
Theoretical weight: 287.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 1-2549; Coverage: 100%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: D, E
Molecule details ›
Chains: D, E
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Regulatory-associated protein of mTOR Chains: W, Y
Molecule details ›
Chains: W, Y
Length: 1343 amino acids
Theoretical weight: 150.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N122 (Residues: 2-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:
Eukaryotic translation initiation factor 4E-binding protein 1 Chains: X, Z
Molecule details ›
Chains: X, Z
Length: 122 amino acids
Theoretical weight: 12.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13541 (Residues: 1-118; Coverage: 100%)
Gene name: EIF4EBP1
Sequence domains: Eukaryotic translation initiation factor 4E binding protein (EIF4EBP)
GTP-binding protein Rheb Chains: R, S
Molecule details ›
Chains: R, S
Length: 188 amino acids
Theoretical weight: 20.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15382 (Residues: 1-184; Coverage: 100%)
Gene names: RHEB, RHEB2
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-7086
Expression system: Homo sapiens