› 6bdz

X-ray diffraction
3.1Å resolution

ADAM10 Extracellular Domain Bound by the 11G2 Fab

Released:
DOI: 10.2210/pdb6bdz/pdb
PDB entry 6bdz coloured by chain, front view.
PDB entry 6bdz coloured by chain, side view.
PDB entry 6bdz coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Regulated Proteolysis by the α-Secretase ADAM10.
Seegar TCM, Killingsworth LB, Saha N, Meyer PA, Patra D, Zimmerman B, Janes PW, Rubinstein E, Nikolov DB, Skiniotis G, Kruse AC, Blacklow SC
Cell 171 1638-1648.e7 (2017)
PMID: 29224781

Function and Biology Details

Reaction catalysed: 
Endopeptidase of broad specificity.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208023 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
11G2 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
11G2 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 24.1 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Disintegrin and metalloproteinase domain-containing protein 10 Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 48.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O14672 (Residues: 220-654; Coverage: 60%)
Gene names: ADAM10, KUZ, MADM
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
3 bound ligands:
Ligand SO4 2 x SO4
Ligand CA 1 x CA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 79.23Å b: 97.58Å c: 262.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.261 0.317
Expression systems:
  • Mus musculus
  • Spodoptera frugiperda

Quick links

Citations

32 review citations

Current understanding of metal ions in the pathogenesis of Alzheimer's disease.
Wang et al. (2020)
31 more

4 mentions without citation

Domain integration of ADAM family proteins: Emerging themes from structural studies.
Seegar et al. (2019)
3 more